UNC13D antibody (Internal Region)

Catalog No. ABIN374716

This Goat Polyclonal antibody specifically detects UNC13D in WB and IHC (p). It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for UNC13D antibody (Internal Region) (ABIN374716)

Target: UNC13D (Unc-13 Homolog D (UNC13D))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This UNC13D antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UNC13D Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts to Munc13-4 / UNC13D.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-ETQKHKKDLHPLFD, from the internal region of the protein sequence

Application Details

Application Notes: ELISA: 1/128000. Western Blot: 1 - 3 μg/mL. Immunohistochemistry on paraffin sections: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for UNC13D

Target: UNC13D (Unc-13 Homolog D (UNC13D))

Alternative Name: UNC13D

Background: Secretion of the contents of cytolytic granules at the immunologic synapse is a highly regulated process essential for lymphocyte cytotoxicity. This process requires the rapid transfer of perforin containing lytic granules to the target cell interface, followed by their docking and fusion with the plasma membrane. Munc 13-4 localises with cytotoxic granules at the immunologic synapse. Munc 13-4 is essential for the priming step of cytolytic granule secretion preceding vesicle membrane fusion. There are two named isoforms.Synonyms: Munc13-4, Protein unc-13 homolog D

Gene ID: 201294, 9606

UniProt: Q70J99

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process