CHRM1 antibody (C-Term)

Catalog No. ABIN374726

The Goat Polyclonal anti-CHRM1 antibody is suitable to detect CHRM1 in samples from Rat, Human, Mouse and Dog. It has been validated for WB.

Quick Overview for CHRM1 antibody (C-Term) (ABIN374726)

Target: CHRM1 (Muscarinic Acetylcholine Receptor M1 (CHRM1))

Reactivity: Rat, Human, Mouse, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This CHRM1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHRM1 Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to M1 mAChR / CHRM1.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-DKRRWRKIPKRPGSVH, from the C Terminus of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/16000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CHRM1

Target: CHRM1 (Muscarinic Acetylcholine Receptor M1 (CHRM1))

Alternative Name: Muscarinic Acetylcholine Receptor M1

Background: The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. Muscarinic receptors are so called as they are more sensitive to the effects of the alkaloid muscarine, rather than nicotine.Synonyms: CHRM1, mAChR M1, mAChR-M1

Gene ID: 1128

NCBI Accession: NP_000729

UniProt: P11229

Pathways: Synaptic Membrane