PIN1 antibody (Internal Region)

Catalog No. ABIN374746

This Goat Polyclonal antibody specifically detects PIN1 in WB. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for PIN1 antibody (Internal Region) (ABIN374746)

Target: PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PIN1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PIN1 Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts to PIN1.

Cross-Reactivity (Details): Species reactivity (expected):Human, Mouse, Canine.
Species reactivity (tested):Rat.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-KHSQSRRPSSWRQEKITR, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/128000. Western Blot: 0.2 - 0.6 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PIN1

Target: PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))

Alternative Name: PIN1

Background: Pin1 is a Peptidyl-prolyl isomerases (PPIase). Peptidyl-prolyl isomerases (PPIase) facilitate the cis-trans interconversion of the peptidyl-prolyl bond thereby affecting protein folding. Pin1 is a PPIase which specifically recognizes phosphorylated S/T-P bonds. Pin1 has been implicated in tau pathologies that underlie Alzheimer's Disease. Pin1 binds to tau phosphorylated specifically on the Thr231-Pro site and induces conformational changes in tau. Such conformational changes can directly restore the ability of phosphorylated Tau to bind microtubules and promote microtubule assembly and/or facilitate tau dephosphorylation. Pin1 expression inversely correlates with the predicted neuronal vulnerability in normally aged brain and also with actual neurofibrillary degeneration in AD brain. Pin1 could be pivotal for maintainance of normal neuronal function and preventing age-dependent neurodegeneration.Synonyms: PIN-1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Peptidyl-prolyl cis-trans isomerase Pin1

Gene ID: 5300

NCBI Accession: NP_006212

UniProt: Q13526