IDE antibody (Internal Region)

Catalog No. ABIN374771

The Goat Polyclonal anti-IDE antibody has been validated for WB. It is suitable to detect IDE in samples from Mouse and Rat.

Quick Overview for IDE antibody (Internal Region) (ABIN374771)

Target: IDE (Insulin-Degrading Enzyme (IDE))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This IDE antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IDE Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts to Insulin-degrading enzyme.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-QQYNYDRDNIE, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/16000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IDE

Target: IDE (Insulin-Degrading Enzyme (IDE))

Alternative Name: Insulin-Degrading Enzyme

Background: Insulysin was identified nearly a century ago as an enzyme responsible for the degradation of insulin in cells, although the precise interactions between insulin and insulysin remain elusive. Human insulysin was cloned in 1988, and shown to be a 118 kDa protein that exists primarily as a homodimer, and perhaps also complexed with other molecules. The sequence is well conserved between humans, rats and mice, and the antibody recognizes these species. Insulysin is a metalloproteinase of the clan ME, family M16, which contains an active site HxxEH, a reversal of the canonical HExxH zinc binding motif. Considered a zinc metalloproteinase, the activity of insulysin can be blocked with EDTA or 1-10 phenanthroline. In addition to the active metalloproteinase domain, insulysin contains a second metalloproteinase site which is considered catalytically inactive, and is thought to assist in substrate binding. Insulysin is most closely related to the bacterial proteinase pitrilysin, (the human orthologue of which appears to be MPRP1) and the mammalian proteinsae nardilysin. Generally thought to be a cytoplasmic protein, insulysin has been isolated from many different tissues and cell lines, and can degrade intact insulin, insulin B chain, glucagon, denatured hemoglobin, alpha amyloid protein, TGF alpha and amylin. Recent work implicates insulysin in clearing beta amyloid plaques from the brain, and has generated much interest in Alzheimer's disease research. The pH optimum for insulysin is basic, pH 8.5, which also distinguishes it from other metalloproteinases. Insulin degrading enzyme (IDE) has a preferential affinity for insulin such that the presence of insulin will inhibit IDE mediated degradation of other substrates. IDE degrades a variety of other peptides including atrial natriuretic peptide and amylin. IDE catabolizes A beta and has been implicated as a candidate enzyme responsible for the degradation and clearance of A beta in the brain. IDE has also been shown to degrade the APP intracellular domain (AICD), a product of gamma secretase cleaved APP that may function in nuclear signaling.Synonyms: Abeta-degrading protease, EC=3.4.24.56, IDE, Insulin protease, Insulinase, Insulysin

Gene ID: 3416

NCBI Accession: NP_004960

UniProt: P14735

Pathways: SARS-CoV-2 Protein Interactome