CELF5 antibody (Internal Region, N-Term)

Catalog No. ABIN374877

The Goat Polyclonal anti-CELF5 antibody (ABIN374877) specifically detects CELF5 in WB. The antibody is reactive with Human samples.

Quick Overview for CELF5 antibody (Internal Region, N-Term) (ABIN374877)

Target: CELF5 (CUGBP, Elav-Like Family Member 5 (CELF5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CELF5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CELF5 Antibody

Binding Specificity: Internal Region, N-Term

Sequence: C-EPPGGQPDGM KD

Specificity: This antibody detects BRUNOL5 / CELF5.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region (near the N Terminus) of the protein sequence according to NP_068757.2 Genename: BRUNOL5

Application Details

Application Notes: Peptide ELISA: limit dilution 1: 1000. Western blot: 1-3 μg/mL. Approx 55 kDa band observed in Human Heart lysates (calculatedMW of 52.5 kDa according to NP_068757.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for CELF5

Target: CELF5 (CUGBP, Elav-Like Family Member 5 (CELF5))

Alternative Name: BRUNOL5 / CELF5

Background: Members of the CELF/BRUNOL protein family contain two N terminal RNA recognition motif (RRM) domains, one C terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre mRNA alternative splicing and may also be involved in mRNA editing, and translation. There are two isoforms.Synonyms: BRUNOL-5, Bruno-like protein 5, CELF-5, CUG-BP- and ETR-3-like factor 5

Gene ID: 60680, 9606

UniProt: Q8N6W0