EWSR1 antibody (Internal Region)

Catalog No. ABIN375098

The Goat Polyclonal anti-EWSR1 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect EWSR1 in samples from Human, Mouse, Rat and Cow.

Quick Overview for EWSR1 antibody (Internal Region) (ABIN375098)

Target: EWSR1 (Ewing Sarcoma Breakpoint Region 1 (EWSR1))

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This EWSR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-EWSR1 Antibody

Binding Specificity: Internal Region

Sequence: C-TSYDQSSYSQ QNTYG

Specificity: This antibody is expected to recognize all five reported isoforms.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Mouse, Rat.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_053733.2, NP_005234.1, NP_001156757.1, NP_001156758.1, NP_001156759.1. Genename: EWSR1

Application Details

Application Notes: Peptide ELISA: 1/16000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for EWSR1

Target: EWSR1 (Ewing Sarcoma Breakpoint Region 1 (EWSR1))

Alternative Name: EWSR1

Background: EWSR1 is a putative RNA binding protein of unknown function. Mutations in this gene, specifically at (11,22)(q24,q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in two products.Synonyms: EWS, EWS oncogene, EWSR-1, Ewing sarcoma breakpoint region 1 protein, RNA-binding protein EWS

Gene ID: 2130, 9606

UniProt: Q01844