DDX19B antibody (Internal Region)

Catalog No. ABIN375191

The Goat Polyclonal anti-DDX19B antibody is suitable to detect DDX19B in samples from Human. It has been validated for WB and EIA.

Quick Overview for DDX19B antibody (Internal Region) (ABIN375191)

Target: DDX19B (DEAD (Asp-Glu-Ala-As) Box Polypeptide 19B (DDX19B))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DDX19B antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-DDX19B Antibody

Binding Specificity: Internal Region

Specificity: This antibody is expected to recognize DDX19A (NP_060802.1) and all reported isoforms of DDX19B.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Mouse and Rat.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography.

Immunogen: Peptide with sequence C-DLPVDKDGNPDNETY, from the internal region of the protein sequence.

Application Details

Application Notes: Peptide ELISA: 1/4000. Western Blot: 0.5-1.5 μg/mL. Approx 50 kDa band observed in lysates of Human ovary and of cell line K562 (CalculatedMW of 50.0 kDa according to NP_001014451.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DDX19B

Target: DDX19B (DEAD (Asp-Glu-Ala-As) Box Polypeptide 19B (DDX19B))

Alternative Name: DDX19B

Background: DDX19B is a DEAD box protein, and an ATP-dependent RNA helicase involved in mRNA export from the nucleus.Synonyms: ATP-dependent RNA helicase DDX19B / DDX19A, DBP5 / DDX19L, DDX19B / DDX19A

Gene ID: 11269

NCBI Accession: NP_001014449

UniProt: Q9UMR2