CD25 antibody (APC)

Catalog No. ABIN375405

This anti-CD25 antibody is a Rat Monoclonal antibody detecting CD25 in FACS. Suitable for Mouse.

Quick Overview for CD25 antibody (APC) (ABIN375405)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD25 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: 7D4

Product Details anti-CD25 Antibody (APC)

Specificity: Mouse CD25, Mr 55 kDa

Characteristics: Rat Anti-Mouse CD25-APC

Purification: Purified

Immunogen: IL-2 dependent BALB/c helper T cell clone, HT2

Isotype: IgM

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Neut - Reported in literature , Block - Reported in literature , Depletion - Reported in literature , CMCD - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE, APC, SPRD, and PE/CY7 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25

Background: The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity α chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. It is transiently expressed at a low level during B-cell development in bone marrow on the CD45R/B220 low TdT - sIg - pre-B II and CD45R/B220 low TdT - sIgM + sIgD - immature B cells, but not on the CD45R/B220 low TdT + sIg - pro-B/pre-B I stage nor on CD45R/B220 high TdT - sIgM + sIgD + mature B cells. CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus.

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation