TNF alpha antibody (Biotin)

Catalog No. ABIN375887

The Rat Monoclonal anti-TNF alpha antibody has been validated for ELISA, FACS and D. It is suitable to detect TNF alpha in samples from Mouse.

Quick Overview for TNF alpha antibody (Biotin) (ABIN375887)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is conjugated to Biotin

Application: ELISA, Flow Cytometry (FACS), Detection (D)

Clone: MP6-XT22

Product Details anti-TNF alpha Antibody (Biotin)

Specificity: Mouse/Chicken TNF-α

Characteristics: Rat Anti-Mouse TNF-a-BIOT

Purification: Purified

Immunogen: E. coli-expressed mouse TNF-alpha

Isotype: IgG1

Application Details

Application Notes:

• Applications: ELISA-Capture - Quality tested , ELISA-Detection - Quality tested , FC - Quality tested , ELISPOT-Capture - Reported in literature , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , WB - Reported in literature , Neut - Reported in literature , Multiplex-Detection - Reported in literature
• Working Dilutions: ELISA Purified (UNLB) antibody ≤ 2 g/mL BIOT conjugate 1:2,000 - 1:4,000 Flow Cytometry FITC conjugate ≤ 1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg labeled antibody in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF-a

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome