ITGA5 antibody (Biotin)

Catalog No. ABIN379959

The Biotin-conjugated Mouse Monoclonal anti-ITGA5 antibody (Clone X-6) (ABIN379959) specifically detects ITGA5 in IHC and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for ITGA5 antibody (Biotin) (ABIN379959)

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA5 antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: X-6

Product Details anti-ITGA5 Antibody (Biotin)

Specificity: The antigen is a 154-180  kDa protein as determined by immunoprecipitation. The epitope has not been further characterized. Antigen distribution: Isolated cells: Granulocytes, monocytes, positive on blood monocyte-derived macrophages days 0-28. Dull staining with 95 % cord blood CD34+ cells, weak reaction on resting neutrophils. Staining increases with maturation of granulocytes. Treatment with fMLP, TNF, GM-CSF and temperature causes positive shift with granulocytes and monocytes. PMA treatment of granulocytes leads to a decrease in the number of positive cells. Positive with cell lines U937, DH39 & MEP, HL-60, NB4, HMC-1. Tissue sections: X-6 is positive on lining cells in different infiltrations and endothelial cells in the synovial membrane of rheumatoid arthritis patients. The staining pattern is similar to CD34 in these patients. Specificity: Human: granulocytes, monocytes, platelets. Other species: not tested.

Purification: Affinity purified

Immunogen: Human monocytes

Type of Immunogen: Cells

Application Details

Application Notes: Approved: IHC, IHC-Fr (1:100)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.5 mL distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS  pH 7.2, 10 mg/mL BSA, 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C.

Target Details for ITGA5

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Alternative Name: ITGA5/Integrin Alpha 5/CD49e

Background: Name/Gene ID: ITGA5
Family: Integrin

Synonyms: ITGA5, FNRA, Integrin alpha-5, Integrin Alpha 5, Integrin alpha-F, VLA-5, CD49e, CD49e antigen, VLA5A

Gene ID: 3678

UniProt: P08648

Pathways: Integrin Complex