GST antibody (HRP)

Catalog No. ABIN387709

This Mouse Monoclonal antibody specifically detects GST in WB and ELISA. It exhibits reactivity toward Schistosoma japonicum.It has been mentioned in 1 publication

Quick Overview for GST antibody (HRP) (ABIN387709)

Target: GST (Glutathione S Transferase (GST))

Reactivity: Schistosoma japonicum

Host: Mouse

Clonality: Monoclonal

Conjugate: This GST antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Clone: 2F10B9

Product Details anti-GST Antibody (HRP)

Specificity: THE^TM GST Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins.

Characteristics: This product is suitable for detecting the expression level of GST in fusion proteins.

Purification: Protein A affinity column

Immunogen: Recombinant GST protein (26 kD)

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05- 0.2 µg/mL
Western blot: 0.1-1 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

References for anti-GST antibody (HRP) (ABIN387709)

Zhang, Mayba, Pfeiffer, Shi, Tepperman, Speed, Quail et al.: "A quartet of PIF bHLH factors provides a transcriptionally centered signaling hub that regulates seedling morphogenesis through differential expression-patterning of shared target genes in ..." in: PLoS genetics, Vol. 9, Issue 1, pp. e1003244, (2013) (PubMed).

Target Details for GST

Target: GST (Glutathione S Transferase (GST))

Alternative Name: Glutathione-S-Transferase (GST)