His Tag antibody (FITC)

Catalog No. ABIN387722

There are 5+ publications for this product available. The Mouse Monoclonal anti-His Tag antibody is suitable to detect His Tag. It has been validated for WB and ELISA.

Quick Overview for His Tag antibody (FITC) (ABIN387722)

Target: His Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is conjugated to FITC

Application: Western Blotting (WB), ELISA

Clone: 6G2A9

Product Details anti-His Tag Antibody (FITC)

Sequence: HHHHHH

Specificity: THE^TM His Tag Antibody [FITC], mAb, Mouserecognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

Characteristics: This product is suitable for detecting the expression level of His fusion proteins by various immunoassays.Absorption Wavelength: 492 nmEmission Wavelength: 518 nmFluorochrome/Protein Ratio: >3 moles FITC per mole antibody

Purification: Protein A affinity column

Immunogen: A synthetic peptide HHHHHH coupled - KLH

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescence (IF): 0.1-2 µg/mL
Flow cytometry: 0.1-2 µg for 1 x 10^6 cells
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-His Tag antibody (FITC) (ABIN387722)

Yang, Wahlmüller, Sarg, Furtmüller, Geiger: "A+-helix of protein C inhibitor (PCI) is a cell-penetrating peptide that mediates cell membrane permeation of PCI." in: The Journal of biological chemistry, Vol. 290, Issue 5, pp. 3081-91, (2015) (PubMed).

Jarmander, Janoschek, Lundh, Larsson, Gustavsson: "Process optimization for increased yield of surface-expressed protein in Escherichia coli." in: Bioprocess and biosystems engineering, Vol. 37, Issue 8, pp. 1685-93, (2014) (PubMed).

An, Budd, Kanost, Michel: "Characterization of a regulatory unit that controls melanization and affects longevity of mosquitoes." in: Cellular and molecular life sciences : CMLS, Vol. 68, Issue 11, pp. 1929-39, (2011) (PubMed).

Oliveira, Lindblad: "Novel insights into the regulation of LexA in the cyanobacterium Synechocystis sp. Strain PCC 6803." in: Journal of bacteriology, Vol. 193, Issue 15, pp. 3804-14, (2011) (PubMed).

Li, Wang, Zhou, Yang, Bhaskar, Hu, Sun, Miao: "Cloning and expression of a cellulase gene in the silkworm, Bombyx mori by improved Bac-to-Bac/BmNPV baculovirus expression system." in: Molecular biology reports, Vol. 37, Issue 8, pp. 3721-8, (2010) (PubMed).

Target Details for His Tag

Target: His Tag

Target Type: Tag