IKBKB antibody

Catalog No. ABIN387826

This Mouse Monoclonal antibody specifically detects IKBKB in WB. It exhibits reactivity toward Human and has been mentioned in 6+ publications.

Quick Overview for IKBKB antibody (ABIN387826)

Target: IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IKBKB antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 62AT216

Product Details anti-IKBKB Antibody

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This IKK beta antibody was raised using purified His-tagged recombinant full length human IKK beta.

Isotype: IgG1

Application Details

Application Notes: WB: 1:100~500. WB: 1:4000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C.

Expiry Date: 6 months

References for anti-IKBKB antibody (ABIN387826)

Boisson, Laplantine, Dobbs, Cobat, Tarantino, Hazen, Lidov, Hopkins, Du, Belkadi, Chrabieh, Itan, Picard, Fournet, Eibel, Tsitsikov, Pai, Abel, Al-Herz, Casanova, Israel, Notarangelo: "Human HOIP and LUBAC deficiency underlies autoinflammation, immunodeficiency, amylopectinosis, and lymphangiectasia." in: The Journal of experimental medicine, Vol. 212, Issue 6, pp. 939-51, (2015) (PubMed).

Tarantino, Tinevez, Crowell, Boisson, Henriques, Mhlanga, Agou, Israël, Laplantine: "TNF and IL-1 exhibit distinct ubiquitin requirements for inducing NEMO-IKK supramolecular structures." in: The Journal of cell biology, Vol. 204, Issue 2, pp. 231-45, (2014) (PubMed).

Boisson, Laplantine, Prando, Giliani, Israelsson, Xu, Abhyankar, Israël, Trevejo-Nunez, Bogunovic, Cepika, MacDuff, Chrabieh, Hubeau, Bajolle, Debré, Mazzolari, Vairo, Agou, Virgin, Bossuyt, Rambaud et al.: "Immunodeficiency, autoinflammation and amylopectinosis in humans with inherited HOIL-1 and LUBAC deficiency. ..." in: Nature immunology, Vol. 13, Issue 12, pp. 1178-86, (2012) (PubMed).

Fan, Meng, Laterra, Rosen: "Role of Src signal transduction pathways in scatter factor-mediated cellular protection." in: The Journal of biological chemistry, Vol. 284, Issue 12, pp. 7561-77, (2009) (PubMed).

Lobry, Lopez, Israël, Weil: "Negative feedback loop in T cell activation through IkappaB kinase-induced phosphorylation and degradation of Bcl10." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 3, pp. 908-13, (2007) (PubMed).

Panopoulos, Harraz, Engelhardt, Zandi et al.: "Iron-mediated H2O2 production as a mechanism for cell type-specific inhibition of tumor necrosis factor alpha-induced but not interleukin-1beta-induced IkappaB kinase complex/nuclear factor-kappaB ..." in: The Journal of biological chemistry, Vol. 280, Issue 4, pp. 2912-23, (2005) (PubMed).

Target Details for IKBKB

Target: IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Alternative Name: IKK beta

Background: NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).

Molecular Weight: 86564

NCBI Accession: NP_001177649, NP_001229707, NP_001547

UniProt: O14920

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins