DNMT3A antibody (C-Term)

Catalog No. ABIN387865

This Rabbit Polyclonal antibody specifically detects DNMT3A in WB. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for DNMT3A antibody (C-Term) (ABIN387865)

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT3A antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB12504

Product Details anti-DNMT3A Antibody

Binding Specificity: AA 723-752, C-Term

Predicted Reactivity: M, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Dnmt3A/Dnmt3A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 723-752 amino acids from the C-terminal region of human Dnmt3A/Dnmt3A2.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-DNMT3A antibody (ABIN387865)

ODoherty, OShea, Sandra, Lonergan, Fair, Forde: "Imprinted and DNA methyltransferase gene expression in the endometrium during the pre- and peri-implantation period in cattle." in: Reproduction, fertility, and development, Vol. 29, Issue 9, pp. 1729-1738, (2018) (PubMed).

ODoherty, OShea, Fair: "Bovine DNA methylation imprints are established in an oocyte size-specific manner, which are coordinated with the expression of the DNMT3 family proteins." in: Biology of reproduction, Vol. 86, Issue 3, pp. 67, (2012) (PubMed).

Target Details for DNMT3A

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Alternative Name: Dnmt3A/Dnmt3A2

Background: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. DNMT3A is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.

Molecular Weight: 101858

Gene ID: 1788

NCBI Accession: NP_072046, NP_715640, NP_783328, NP_783329

UniProt: Q9Y6K1