JMJD4 antibody (C-Term)

Catalog No. ABIN387875

The Rabbit Polyclonal anti-JMJD4 antibody has been validated for WB and IHC (p). It is suitable to detect JMJD4 in samples from Human and Mouse.

Quick Overview for JMJD4 antibody (C-Term) (ABIN387875)

Target: JMJD4 (Jumonji Domain Containing 4 (JMJD4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JMJD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB10802

Product Details anti-JMJD4 Antibody

Binding Specificity: AA 423-455, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This JMJD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423-455 amino acids from the C-terminal region of human JMJD4.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for JMJD4

Target: JMJD4 (Jumonji Domain Containing 4 (JMJD4))

Alternative Name: JMJD4

Background: Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.

Molecular Weight: 52493

Gene ID: 65094

NCBI Accession: NP_001154937, NP_075383

UniProt: Q9H9V9