DNMT1 antibody (C-Term)

Catalog No. ABIN387878

The Rabbit Polyclonal anti-DNMT1 antibody has been validated for WB and FACS. It is suitable to detect DNMT1 in samples from Human. There are 2+ publications available.

Quick Overview for DNMT1 antibody (C-Term) (ABIN387878)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: RB01848

Product Details anti-DNMT1 Antibody

Binding Specificity: AA 1588-1616, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Dnmt1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1588-1616 amino acids from the C-terminal region of human Dnmt1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-DNMT1 antibody (ABIN387878)

Bing, Zhu, Yu, Li, Liu, Li, Wang, Qi, Guo, Yuan, He, Liu, Liu: "Glucocorticoid-induced S-adenosylmethionine enhances the interferon signaling pathway by restoring STAT1 protein methylation in hepatitis B virus-infected cells." in: The Journal of biological chemistry, Vol. 289, Issue 47, pp. 32639-55, (2014) (PubMed).

Li, Weyand, Goronzy: "Epigenetic mechanisms of age-dependent KIR2DL4 expression in T cells." in: Journal of leukocyte biology, Vol. 84, Issue 3, pp. 824-34, (2008) (PubMed).

Target Details for DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: DNMT1

Background: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2Fbinding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.

Molecular Weight: 183165

Gene ID: 1786

NCBI Accession: NP_001124295, NP_001370

UniProt: P26358

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection