His Tag antibody

Details for Product No. ABIN387901, Supplier: Log in to see
Antigen
Reactivity
Tag
623
28
15
5
4
1
Host
Rabbit
416
187
27
23
8
8
3
2
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
58
46
37
17
14
13
10
10
10
9
9
9
8
8
8
7
7
7
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
570
405
164
110
83
82
79
27
13
11
8
7
6
6
6
5
4
4
4
4
2
2
2
1
1
1
1
1
1
1
1
1
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Immunogen Poly-HIS peptide were used to produced this antibody.
Sequence HHHHHH
Clone RB19955
Isotype Ig Fraction
Specificity Poly-HIS peptide were used to produced this antibody.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name 6 X His Tag
Target Type Tag
Background Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
Synonyms: Poly-His, Hexa-HIS, Hexa-Histidine
Molecular Weight 1000 DA
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-His Tag antibody (ABIN387901) Western blot analysis of 6*His Tag Antibody (ABIN387901) in his-tag TBB1 protein (35 ...
Product cited in: Anderson, Robson, Jiang, Malmirchegini, Fierobe, Lazazzera, Clubb: "Assembly of minicellulosomes on the surface of Bacillus subtilis." in: Applied and environmental microbiology, Vol. 77, Issue 14, pp. 4849-58, 2011 (PubMed).

Background publications Hochuli, Döbeli, Schacher: "New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues." in: Journal of chromatography, Vol. 411, pp. 177-84, 1988 (PubMed).

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