His Tag antibody

Catalog No. ABIN387901

This Rabbit Polyclonal antibody specifically detects His Tag in WB. It has been mentioned in 1 publication

Quick Overview for His Tag antibody (ABIN387901)

Target: His Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB19955

Product Details anti-His Tag Antibody

Sequence: HHHHHH

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: Poly-HIS peptide were used to produced this antibody.

Isotype: IgG

Application Details

Application Notes: WB: 1:2000. WB: 1:2000. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-His Tag antibody (ABIN387901)

Anderson, Robson, Jiang, Malmirchegini, Fierobe, Lazazzera, Clubb: "Assembly of minicellulosomes on the surface of Bacillus subtilis." in: Applied and environmental microbiology, Vol. 77, Issue 14, pp. 4849-58, (2011) (PubMed).

Target Details for His Tag

Target: His Tag

Alternative Name: 6 X His Tag

Target Type: Tag

Background: Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.