SUMO4 antibody (Mutant, Val55)

Catalog No. ABIN388079

The Rabbit Polyclonal anti-SUMO4 antibody has been validated for WB and IHC (p). It is suitable to detect SUMO4 in samples from Human.

Quick Overview for SUMO4 antibody (Mutant, Val55) (ABIN388079)

Target: SUMO4 (Small Ubiquitin Related Modifier 4 (SUMO4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUMO4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB7264

Product Details anti-SUMO4 Antibody

Binding Specificity: Mutant, Val55

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SUMO4 antibody is generated from rabbits immunized with a KLH conjugated peptide CEPRGLS(V)KQIRFRFG selected from human SUMO4. This antibody is affinity purified using peptides CEPRGLS(V)KQIRFRFG (positive selection) and CEPRGLS(M)KQIRFRFG (negative selection).

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for SUMO4

Target: SUMO4 (Small Ubiquitin Related Modifier 4 (SUMO4))

Alternative Name: SUMO4

Background: SUMO4 is a member of the SUMO gene family. This family of small ubiquitin-related modifiers covalently modify target lysines in proteins and control the target proteins' subcellular localization, stability, or activity. Upon oxidative stress, SUMO4 conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. This protein may also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity. Covalent attachment to SUMO4 substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I. In contrast to SUMO1, SUMO2 and SUMO3, SUMO4 seems to be insensitive to sentrin-specific proteases due to the presence of Pro-90. This may impair processing to mature form and conjugation to substrates. SUMO4 is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-kappa-B-dependent transcription of the IL12B gene. The M55V substitution has been associated with type I diabetes.

Molecular Weight: 10685

Gene ID: 387082

NCBI Accession: NP_001002255

UniProt: Q6EEV6