MCL-1 antibody (AA 191-226)

Catalog No. ABIN388105

This Rabbit Polyclonal antibody specifically detects MCL-1 in WB, FACS and IHC (p). It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 4+ publications.

Quick Overview for MCL-1 antibody (AA 191-226) (ABIN388105)

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCL-1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB00961

Product Details anti-MCL-1 Antibody

Binding Specificity: AA 191-226

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This MCL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-226 amino acids from human MCL1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. WB: 1:2000. IHC-P: 1:10~50. FC: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-MCL-1 antibody (ABIN388105)

Ma, He, Zhang, Fei, Niu, Wang, Ding, Xu, Chen, Zhu: "DNA methylation-regulated miR-193a-3p dictates resistance of hepatocellular carcinoma to 5-fluorouracil via repression of SRSF2 expression." in: The Journal of biological chemistry, Vol. 287, Issue 8, pp. 5639-49, (2012) (PubMed).

Tsang, Tang, Chan, Co, Au Yeung, Yau, Kong, Fung, Kwok: "P-glycoprotein enhances radiation-induced apoptotic cell death through the regulation of miR-16 and Bcl-2 expressions in hepatocellular carcinoma cells." in: Apoptosis : an international journal on programmed cell death, Vol. 16, Issue 5, pp. 524-35, (2011) (PubMed).

Johnson, Lucas, Muthusamy, Smith, Edwards, De Lay, Croce, Grever, Byrd: "Characterization of the TCL-1 transgenic mouse as a preclinical drug development tool for human chronic lymphocytic leukemia." in: Blood, Vol. 108, Issue 4, pp. 1334-8, (2006) (PubMed).

Yamane, Dylla, Muijtjens, Weissman: "Enforced Bcl-2 expression overrides serum and feeder cell requirements for mouse embryonic stem cell self-renewal." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 9, pp. 3312-7, (2005) (PubMed).

Target Details for MCL-1

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Alternative Name: MCL1

Background: The Mcl-1 protein belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.

Molecular Weight: 37337

Gene ID: 4170

NCBI Accession: NP_001184249, NP_068779, NP_877495

UniProt: Q07820

Pathways: MAPK Signaling