BAP1 antibody (AA 36-66)

Catalog No. ABIN388948

This Rabbit Polyclonal antibody specifically detects BAP1 in WB, IF, IHC (p) and FACS. It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for BAP1 antibody (AA 36-66) (ABIN388948)

Target: BAP1 (BRCA1 Associated Protein-1 (Ubiquitin Carboxy-terminal Hydrolase) (BAP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: RB4559-4560

Product Details anti-BAP1 Antibody

Binding Specificity: AA 36-66

Predicted Reactivity: B, Zf

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This BAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-66 amino acids of human BAP1.

Isotype: IgG

Application Details

Application Notes: IF: 1:25. WB: 1:2000. WB: 1:1000. IHC-P: 1:100. IHC-P: 1:100. FC: 1:25. IHC: 1:250

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C.

Expiry Date: 6 months

References for anti-BAP1 antibody (ABIN388948)

Abdel-Rahman, Pilarski, Cebulla, Massengill, Christopher, Boru, Hovland, Davidorf: "Germline BAP1 mutation predisposes to uveal melanoma, lung adenocarcinoma, meningioma, and other cancers." in: Journal of medical genetics, Vol. 48, Issue 12, pp. 856-9, (2011) (PubMed).

Target Details for BAP1

Target: BAP1 (BRCA1 Associated Protein-1 (Ubiquitin Carboxy-terminal Hydrolase) (BAP1))

Alternative Name: BAP1

Background: 'BRCA1-associated protein-1,' or BAP1 interacts with the RING finger domain of BRCA1. The N-terminal 240 amino acids of the predicted 729-amino acid human protein show homology to ubiquitin C-terminal hydrolases (UCHs), thiol proteases that catalyze proteolytic processing of ubiquitin. In addition, BAP1 contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals.. BAP1 and BRCA1 associate in vivo and have overlapping subnuclear localization patterns.1 BAP1 enhances BRCA1-mediated inhibition of breast cancer cell growth. Northern blot analysis indicates that BAP1 is expressed as a 4-kb mRNA in all human tissues tested, with A 4.8-kb transcript expressed exclusively in testis. Northern blot analysis and in situ hybridization reveal that BAP1 and BRCA1 are coexpressed during murine breast development and remodeling. The BAP1 gene has been mapped to 3p21.3, a region of loss of heterozygosity for breast cancer as well as frequently deleted in lung carcinomas.1 Intragenic homozygous rearrangements and deletions of BAP1 appear in lung carcinoma cell lines. It has been postulated that BAP1 is a tumor suppressor gene that functions in the BRCA1 growth control pathway.1

Molecular Weight: 80362

NCBI Accession: NP_004647

UniProt: Q92560