Cyclin D1 antibody (AA 147-175)

Catalog No. ABIN389145

This anti-Cyclin D1 antibody is a Rabbit Polyclonal antibody detecting Cyclin D1 in WB. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Cyclin D1 antibody (AA 147-175) (ABIN389145)

Target: Cyclin D1 (CCND1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB14995

Product Details anti-Cyclin D1 Antibody

Binding Specificity: AA 147-175

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This CCND1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the Central region of human CCND1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-Cyclin D1 antibody (ABIN389145)

Ling, Song, Fan, Feng, Liu, Yang, Wang, Li, Tian, Zhao, Liu, Huang, Hou, Xu, Shi, Li: "Combination of metformin and sorafenib suppresses proliferation and induces autophagy of hepatocellular carcinoma via targeting the mTOR pathway." in: International journal of oncology, Vol. 50, Issue 1, pp. 297-309, (2017) (PubMed).

Ling, Feng, Ke, Fan, Li, Li, Dong, Wang, Xu, Li, Wang: "Metformin inhibits proliferation and enhances chemosensitivity of intrahepatic cholangiocarcinoma cell lines." in: Oncology reports, Vol. 31, Issue 6, pp. 2611-8, (2014) (PubMed).

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: CCND1

Background: CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of the gene encoding this protein, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.

Molecular Weight: 33729

Gene ID: 595

UniProt: Q6FI00

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling