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Vimentin antibody (AA 152-181)

Name: Vimentin antibody (AA 152-181)
SKU: ABIN389213
Price: 451 USD
Availability: InStock

The Rabbit Polyclonal anti-Vimentin antibody has been validated for WB, FACS and IHC (p). It is suitable to detect Vimentin in samples from Human.

Western blot analysis of Vimentin Antibody (Center) (ABIN389213 and ABIN2839368) in , cell line lysates (35 μg/lane). (Vimentin antibody (AA 152-181))

Vimentin Antibody (Center) (ABIN389213 and ABIN2839368) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). (Vimentin antibody (AA 152-181))

FACS

Western blot analysis of Vimentin (arrow) using Vimentin Antibody (Center) (ABIN389213 and ABIN2839368). (Vimentin antibody (AA 152-181))

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (Vimentin antibody (AA 152-181))

IHC (p)

Vimentin Antibody (Center) (ABIN389213 and ABIN2839368) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. (Vimentin antibody (AA 152-181))

IHC (p)

5 images

Catalog No. ABIN389213

Datasheet as PDF

Quick Overview for Vimentin antibody (AA 152-181) (ABIN389213)

Target

See all Vimentin (VIM) Antibodies

Vimentin (VIM)

Reactivity

Human

Host

Rabbit

Clonality

Polyclonal

Conjugate

This Vimentin antibody is un-conjugated

Application

Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

RB15676

Discover our top product VIM Primary Antibody

Binding Specificity
32

24

17

16

12

10

9

7

5

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 152-181

Predicted Reactivity

B, Ha, M, Pig, Rat

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen

This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-181 amino acids from the Central region of human Vimentin.

Isotype

Ig Fraction
anti-Vimentin (VIM) (AA 371-466) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, IF Host: Mouse Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, IF, IP Host: Rabbit Monoclonal unconjugated

Application Notes

WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50. IHC-P: 1:10~50. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date

6 months
Target

Vimentin (VIM)

Alternative Name

Vimentin

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Molecular Weight

53652

Gene ID

7431

NCBI Accession

NP_003371

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis