CDC25A antibody (pThr507)

Catalog No. ABIN389532

Cited in 2+ publications. The Rabbit Polyclonal anti-CDC25A antibody (Clone RB7856) (ABIN389532) specifically detects CDC25A in WB, IF and IHC (p). The antibody is reactive with Human samples.

Quick Overview for CDC25A antibody (pThr507) (ABIN389532)

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB7856

Product Details anti-CDC25A Antibody

Binding Specificity: AA 480-514, pThr507

Predicted Reactivity: B, M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This CDC25A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T507 of human CDC25A.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:25. WB: 1:250. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C.

Expiry Date: 6 months

References for anti-CDC25A antibody (ABIN389532)

Chou, Yen, Lee, Chen: "Pro-apoptotic role of Cdc25A: activation of cyclin B1/Cdc2 by the Cdc25A C-terminal domain." in: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17833-45, (2010) (PubMed).

Kasahara, Goto, Enomoto, Tomono, Kiyono, Inagaki: "14-3-3gamma mediates Cdc25A proteolysis to block premature mitotic entry after DNA damage." in: The EMBO journal, Vol. 29, Issue 16, pp. 2802-12, (2010) (PubMed).

Target Details for CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name: CDC25A

Background: CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 59087

NCBI Accession: NP_001780, NP_963861

UniProt: P30304

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase