MEK1 antibody (pSer218, pSer222)

Catalog No. ABIN389590

This Rabbit Polyclonal antibody specifically detects MEK1 in WB, IHC (p) and DB. It exhibits reactivity toward Human.

Quick Overview for MEK1 antibody (pSer218, pSer222) (ABIN389590)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Clone: RB05985

Product Details anti-MEK1 Antibody

Binding Specificity: pSer218, pSer222

Predicted Reactivity: D, E, Ha, M, Rb, Rat, X, C

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This MEK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S218/222 of human MEK1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:500. IHC-P: 1:50~100. DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MEK1

Background: MEK1is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.

Molecular Weight: 43439

Gene ID: 5604

NCBI Accession: NP_002746

UniProt: Q02750

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling