WEE1 antibody (pSer123)

Catalog No. ABIN2839651

There is 1 publication for this product available. The Rabbit Polyclonal anti-WEE1 antibody is suitable to detect WEE1 in samples from Human. It has been validated for IHC (p) and DB.

Quick Overview for WEE1 antibody (pSer123) (ABIN2839651)

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WEE1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Clone: RB07865

Product Details anti-WEE1 Antibody

Binding Specificity: pSer123

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Wee1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S123 of human Wee1.

Isotype: Ig Fraction

Application Details

Application Notes: IHC-P: 1:50~100. DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-WEE1 antibody (ABIN2839651)

Müller, Lutter, Püschel: "Persistence of the cell-cycle checkpoint kinase Wee1 in SadA- and SadB-deficient neurons disrupts neuronal polarity." in: Journal of cell science, Vol. 123, Issue Pt 2, pp. 286-94, (2010) (PubMed).

Target Details for WEE1

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Alternative Name: Wee1

Background: This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

Molecular Weight: 71597

Gene ID: 7465

NCBI Accession: NP_001137448, NP_003381

UniProt: P30291

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase