p53 antibody (C-Term)

Catalog No. ABIN390199

This Rabbit Polyclonal antibody specifically detects p53 in WB. It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for p53 antibody (C-Term) (ABIN390199)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB07832

Product Details anti-p53 Antibody

Binding Specificity: AA 354-385, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This p53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 354-385 amino acids from the C-terminal region of human p53.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000-1:2000. WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-p53 antibody (ABIN390199)

Manu, Chai, Teh, Zhu, Casey, Wang: "Inhibition of Isoprenylcysteine Carboxylmethyltransferase Induces Cell-Cycle Arrest and Apoptosis through p21 and p21-Regulated BNIP3 Induction in Pancreatic Cancer." in: Molecular cancer therapeutics, Vol. 16, Issue 5, pp. 914-923, (2018) (PubMed).

Qiang, Sui, Ma, Li, Ren, Shao, Liu, Guan, Shi, Hou: "Proteasome inhibitor MG132 induces thyroid cancer cell apoptosis by modulating the activity of transcription factor FOXO3a." in: Endocrine, Vol. 56, Issue 1, pp. 98-108, (2017) (PubMed).

Huang, Wu, Mei, Wu: "XIAP inhibits autophagy via XIAP-Mdm2-p53 signalling." in: The EMBO journal, Vol. 32, Issue 16, pp. 2204-16, (2013) (PubMed).

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53

Background: Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.

Molecular Weight: 43653

Gene ID: 7157

NCBI Accession: NP_000537, NP_001119584, NP_001119585, NP_001119586, NP_001119587, NP_001119588, NP_001119589, NP_001119590, NP_001263624, NP_001263625, NP_001263626, NP_001263627, NP_001263628, NP_00126

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect