Glypican 3 antibody (N-Term)

Catalog No. ABIN390317

The Rabbit Polyclonal anti-Glypican 3 antibody has been validated for WB and IHC (p). It is suitable to detect Glypican 3 in samples from Human and Mouse. There is 1 publication available.

Quick Overview for Glypican 3 antibody (N-Term) (ABIN390317)

Target: Glypican 3 (GPC3)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glypican 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB05031

Product Details anti-Glypican 3 Antibody

Binding Specificity: AA 21-50, N-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Glypican 3 (GPC3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human Glypican 3 (GPC3).

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. WB: 1:2000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-Glypican 3 antibody (ABIN390317)

Han, Ma, Zhao, Zhao, Batista, Zhou, Zhou, Yang, Wang, Bi, Xia, Bai, Garkavtsev, Zhang: "Identification of Glypican-3 as a potential metastasis suppressor gene in gastric cancer." in: Oncotarget, Vol. 7, Issue 28, pp. 44406-44416, (2018) (PubMed).

Target Details for Glypican 3

Target: Glypican 3 (GPC3)

Background: GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in the control of cell division, growth regulation, and tumor predisposition. Deletion mutations in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS), also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.

Molecular Weight: 65563

Gene ID: 2719

NCBI Accession: NP_001158089, NP_001158091, NP_004475

UniProt: P51654

Pathways: Glycosaminoglycan Metabolic Process