TBP antibody (C-Term)

Catalog No. ABIN2840934

The Rabbit Polyclonal anti-TBP antibody (Clone RB20260) (ABIN2840934) specifically detects TBP in WB, IF and IHC (p). The antibody is reactive with Human samples.

Quick Overview for TBP antibody (C-Term) (ABIN2840934)

Target: TBP (TATA Box Binding Protein (TBP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB20260

Product Details anti-TBP Antibody

Binding Specificity: AA 301-330, C-Term

Predicted Reactivity: B, C, Pr, M, X

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This TBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-330 amino acids from the C-terminal region of human TBP.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for TBP

Target: TBP (TATA Box Binding Protein (TBP))

Alternative Name: TBP

Background: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.

Molecular Weight: 37698

Gene ID: 6908

NCBI Accession: NP_001165556, NP_003185

UniProt: P20226

Pathways: WNT Signaling