Cyclin A antibody (N-Term)

Catalog No. ABIN391454

The Rabbit Polyclonal anti-Cyclin A antibody has been validated for WB, FACS and IHC (p). It is suitable to detect Cyclin A in samples from Human. There is 1 publication available.

Quick Overview for Cyclin A antibody (N-Term) (ABIN391454)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin A antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB14617

Product Details anti-Cyclin A Antibody

Binding Specificity: AA 51-84, N-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Cyclin A (CCNA2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-84 amino acids from the N-terminal region of human Cyclin A (CCNA2).

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-Cyclin A antibody (ABIN391454)

Chang, Nam, Kook, Kim, Liu, Yao, Jung, Lemos, Seo, Park, Gim, Hong, Huh, Kim, Tan, Liu, Powis, Park, Liang, Kim: "HNF4? is a therapeutic target that links AMPK to WNT signalling in early-stage gastric cancer." in: Gut, (2014) (PubMed).

Target Details for Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Alternative Name: Cyclin A (CCNA2)

Background: CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

Molecular Weight: 48551

Gene ID: 890

NCBI Accession: NP_001228

UniProt: P20248

Pathways: PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA