DGKB antibody (C-Term)

Catalog No. ABIN392727

Cited in 1 publication. The Rabbit Polyclonal anti-DGKB antibody (Clone RB3877) (ABIN392727) specifically detects DGKB in WB and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for DGKB antibody (C-Term) (ABIN392727)

Target: DGKB (Diacylglycerol Kinase, beta 90kDa (DGKB))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGKB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB3877

Product Details anti-DGKB Antibody

Binding Specificity: AA 773-804, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This DGKB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 773-804 amino acids from the C-terminal region of human DGKB.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-DGKB antibody (ABIN392727)

Dorrell, Grompe, Pan, Zhong, Canaday, Shultz, Greiner, Wright, Streeter, Grompe: "Isolation of mouse pancreatic alpha, beta, duct and acinar populations with cell surface markers." in: Molecular and cellular endocrinology, Vol. 339, Issue 1-2, pp. 144-50, (2011) (PubMed).

Target Details for DGKB

Target: DGKB (Diacylglycerol Kinase, beta 90kDa (DGKB))

Alternative Name: DGKB

Background: Diacylglycerol (DAG) is an allosteric activator of protein kinase C. DAG also participates in regulating RAS and RHO family proteins by activating the guanine nucleotide exchange factors VAV and RASGRP1. DAG is also involved in the synthesis of phospholipids and triacylglycerols. Tight regulation of DAG levels is achieved via DAG kinases (DGKs), which remove DAG by phosphorylate it to phosphatidic acid. Several mammalian isozymes of DAGK have been identified

Molecular Weight: 90595

Gene ID: 1607

NCBI Accession: NP_004071, NP_663733

UniProt: Q9Y6T7