DGKB antibody (AA 630-710)

Catalog No. ABIN7219954

The Rabbit Polyclonal anti-DGKB antibody is suitable to detect DGKB in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for DGKB antibody (AA 630-710) (ABIN7219954)

Target: DGKB (Diacylglycerol Kinase, beta 90kDa (DGKB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGKB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DGKB Antibody

Binding Specificity: AA 630-710

Purpose: Rabbit Anti-DGK-β Polyclonal Antibody

Specificity: DGK-β Polyclonal Antibody detects endogenous levels of DGK-β protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human DGK-beta at AA range: 630-710

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DGKB

Target: DGKB (Diacylglycerol Kinase, beta 90kDa (DGKB))

Alternative Name: DGK-beta

Background: DGKB, DAGK2, KIAA0718, Diacylglycerol kinase beta, DAG kinase beta, 90 kDa diacylglycerol kinase, Diglyceride kinase beta, DGK-betaDiacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). Diacylglycerol kinase beta encoded by DGKB is a diacylglycerol kinase, beta isotype. Two alternatively spliced transcript variants have been found for this gene.

Molecular Weight: 90kD

Gene ID: 1607

UniProt: Q9Y6T7