DLG4 antibody (C-Term)

Catalog No. ABIN396947

The Rabbit Polyclonal anti-DLG4 antibody has been validated for WB, IHC, ELISA, IF, IP and IHC (p). It is suitable to detect DLG4 in samples from Rat, Human and Mouse.

Quick Overview for DLG4 antibody (C-Term) (ABIN396947)

Target: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Reactivity: Rat, Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLG4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DLG4 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Recognizes the ~95kD PSD-95 protein. Cross-reactivity with an unknown protein at ~50kD with rat brain homogenates but not with mouse brain homogenates. Species cross-reactivity: human, rat and mouse.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the C-terminal region of human PSD-95 (KLH).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (0.5 - 1 μg/mL), IHC, IHC-P (5 μg/mL), IP, WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for DLG4

Target: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Alternative Name: DLG4 / PSD95

Background: Name/Gene ID: DLG4
Family: MAGUK

Synonyms: DLG4, Disks large homolog 4, Presynaptic density protein 95, PSD95, SAP-90, Synapse-associated protein 90, PSD-95, SAP90, Tax interaction protein 15, Discs large homolog 4

Gene ID: 1742

UniProt: P78352

Pathways: Regulation of Muscle Cell Differentiation, Synaptic Membrane, Skeletal Muscle Fiber Development, Asymmetric Protein Localization, Regulation of long-term Neuronal Synaptic Plasticity