CENPF antibody (AA 1759-2093)

Catalog No. ABIN396951

The Mouse Monoclonal anti-CENPF antibody (ABIN396951) specifically detects CENPF in IHC, WB, IHC (p), IP and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for CENPF antibody (AA 1759-2093) (ABIN396951)

Target: CENPF (Centromere Protein F (CENPF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CENPF antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CENPF Antibody

Binding Specificity: AA 1759-2093

Specificity: Recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.

Purification: Protein G purified

Immunogen: GST fusion protein expressed in E. coli corresponding to aa 1759-2093 of human mitosin.

Type of Immunogen: Fusion protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: IHC, IHC-Fr (10 μg/mL), IHC-P (10 μg/mL), IP (1 - 10 μg/mL), WB (1 - 10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: 1-10 μg/mL. Immunoprecipitation: 1-10 μg/mL. Immunohistochemistry: 10 μg/mL with frozen or paraffin-embedded sections. Tested in IHC using formalin-fixed paraffin-embedded human tissues, HIER antigen retrieval using sodium citrate buffer at  pH 6.0.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4

Handling Advice: Avoid freeze/thaw cycles.

Storage: -80 °C

Storage Comment: Store at -70°C, avoid freeze/thaw cycles.

Target Details for CENPF

Target: CENPF (Centromere Protein F (CENPF))

Alternative Name: CENPF / CENP-F

Background: Name/Gene ID: CENPF

Synonyms: CENPF, AH antigen, Centromere protein F, CENF, CENP-F, Kinetochore protein CENPF, Mitosin, CENP-F kinetochore protein, Hcp-1, PRO1779

Gene ID: 1063

UniProt: P49454

Pathways: Chromatin Binding, M Phase, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection