Granulin antibody (C-Term)

Catalog No. ABIN396952

This Rabbit Polyclonal antibody specifically detects Granulin in WB, IHC, IP and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Granulin antibody (C-Term) (ABIN396952)

Target: Granulin (GRN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Granulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Granulin Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Recognizes human PCDGF protein at ~64-88kD.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the C-terminal region of human PCDGF (PC cell-derived growth factor, GP88, acrogranin, granulin precursor, epithelin precursor, or progranulin).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: Human MDA-MB-231 breast adenocarcinoma, PC-3 prostate adenocarcinoma (bone metastatic site), HeLa cervical carcinoma, and HT1080 fibrosarcoma lysates.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Granulin

Target: Granulin (GRN)

Alternative Name: GRN / Progranulin / Granulin

Background: Name/Gene ID: GRN
Family: Hormone

Synonyms: GRN, Acrogranin, CLN11, Epithelin precursor, GEP, gp88, Granulin, Granulin-epithelin, Granulins, PC cell-derived growth factor, PCDGF, PEPI, PGRN, Proepithelin, Progranulin

Gene ID: 2896

UniProt: P28799