HYOU1 antibody (C-Term)

Catalog No. ABIN396969

This anti-HYOU1 antibody is a Rabbit Polyclonal antibody detecting HYOU1 in WB, IHC, ELISA and IHC (p). Suitable for Human and Rat.

Quick Overview for HYOU1 antibody (C-Term) (ABIN396969)

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HYOU1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HYOU1 Antibody

Binding Specificity: C-Term

Specificity: Synthetic peptide corresponding to C-terminal residues of rat Hyou1(Hypoxia up-regulated 1) or Orp150 (150 kD oxygen-regulated protein precursor).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from rat HYOU1 / ORP150.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.01 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for HYOU1

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Alternative Name: HYOU1 / ORP150

Background: Name/Gene ID: HYOU1

Synonyms: HYOU1, GRP-170, Grp170, Glucose-regulated protein 170, Hypoxia up-regulated 1, Hypoxia up-regulated protein 1, HSP12A, ORP150, ORP-150

Gene ID: 10525

UniProt: Q9Y4L1

Pathways: ER-Nucleus Signaling, SARS-CoV-2 Protein Interactome