CDK2 antibody

Catalog No. ABIN400761

This anti-CDK2 antibody is a Mouse Monoclonal antibody detecting CDK2 in WB, IP, IHC (p) and EIA. Suitable for Human, Mouse and Xenopus laevis.

Quick Overview for CDK2 antibody (ABIN400761)

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reactivity: Human, Mouse, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 4i33

Product Details anti-CDK2 Antibody

Specificity: Recognizes Human CDK2 (MW 33 kDa). Does not cross-react with other CDKs.

Cross-Reactivity (Details): Species reactivity (expected):Xenopus and Mouse.
Species reactivity (tested):Human.

Characteristics: Synonyms: p33 protein kinase, Cell division protein kinase 2, Cyclin dependent kinase 2

Purification: Protein G Chromatography

Immunogen: Recombinant Human CDK2.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.85 mg/mL

Buffer: 0.1 M Tris-glycine, pH 7.4, 150 mM Sodium Chloride, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Alternative Name: CDK2

Background: Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.Synonyms: Cell division protein kinase 2, Cyclin dependent kinase 2, p33 protein kinase

Gene ID: 1017

UniProt: P24941

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA