MARK2 antibody (Isoform A, pThr595)

Catalog No. ABIN401318

The Rabbit Polyclonal anti-MARK2 antibody is suitable to detect MARK2 in samples from Human, Mouse, Rat, Cow and Dog. It has been validated for WB and EIA.

Quick Overview for MARK2 antibody (Isoform A, pThr595) (ABIN401318)

Target: MARK2 (MAP/microtubule Affinity-Regulating Kinase 2 (MARK2))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARK2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-MARK2 Antibody

Binding Specificity: Isoform A, pThr595

Specificity: This antibody is directed against the phosphorylated form of MARK2 at thepT595 residue.

Cross-Reactivity (Details): Species reactivity (expected):Canine, Bovine, Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids surrounding T595 of Human MARK2 isoform a. The immunogen peptide was phosphorylated at the residue corresponding to T595

Isotype: IgG

Application Details

Application Notes: ELISA: 1/1,000 - 1/3,000. Western Blot: 1/250 - 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.6 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for MARK2

Target: MARK2 (MAP/microtubule Affinity-Regulating Kinase 2 (MARK2))

Alternative Name: MARK2

Background: This antibody recognizes a regulatory phosphorylation on a kinase that is critical to regulation of microtubules, cell cycle and immune responses. MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encode two isoforms of the human ser/thr protein kinase EMK1 called MARK2. These isoforms were characterized by the presence of a 162-bp alternative exon that gives rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be co-expressed in a number of selected cell lines and tissue samples. Human MARK2 was shown to be encoded by a single mRNA that is ubiquitously expressed. This transcription variant includes the alternative exon in the coding region and therefore codes for a longer protein. Multiple splice variants exist for this protein.Synonyms: 2, ELKL motif kinase, EMK1, MAP/microtubule affinity-regulating kinase, MARK2, PAR1 homolog, Serine/threonine-protein kinase

Gene ID: 2011

NCBI Accession: NP_001034558

UniProt: Q7KZI7

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection