RAB11FIP3 antibody (C-Term)

Catalog No. ABIN401388

This anti-RAB11FIP3 antibody is a Rabbit Polyclonal antibody detecting RAB11FIP3 in WB and EIA. Suitable for Human, Mouse and Rat.

Quick Overview for RAB11FIP3 antibody (C-Term) (ABIN401388)

Target: RAB11FIP3 (RAB11 Family Interacting Protein 3 (Class II) (RAB11FIP3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB11FIP3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-RAB11FIP3 Antibody

Binding Specificity: C-Term

Specificity: This antibody is directed against FIP3 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Recombinant protein corresponding to amino acids spanning the carboxyl terminal one- third of human FIP3

Isotype: IgG

Application Details

Application Notes: ELISA: 1/1,000 - 1/3,000. Western Blot: 1/200 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.19 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for RAB11FIP3

Target: RAB11FIP3 (RAB11 Family Interacting Protein 3 (Class II) (RAB11FIP3))

Alternative Name: RAB11FIP3

Background: Rab11 Family-Interacting Protein 3 (FIP3)/Arfophilin-1 and FIP4/Arfophilin-2 are dual effectors for Rab11 and the ADP-ribosylation factor Arf6. Rab11 and Arf6 are involved in membrane delivery from recycling endosomes to the plasma membrane during cytokinesis. The dual Rab11/Arf6 binding proteins, FIP3 and FIP4, function in the delivery of recycling endosomes to the cleavage furrow and are, together with Rab11, essential for completion of abscission, the terminal step of cytokinesis. An interaction between Rab11-FIP3 and ASAP1 has also been identified (personal communication, P. Randazzo, CCR-NCI, Bethesda, MD). ASAP1 is an Arf GTPase-activating protein that has been implicated in the invasive properties of uveal melanoma and mammary carcinoma.Synonyms: ARFO1, Arfophilin-1, Eferin, FIP3-Rab11, KIAA0665, MU-MB-17.148, Rab11 family-interacting protein 3, Rab11-FIP3

Gene ID: 9727

NCBI Accession: NP_001135744

UniProt: O75154