TDGF1 antibody (Internal Region)

Catalog No. ABIN401391

This anti-TDGF1 antibody is a Rabbit Polyclonal antibody detecting TDGF1 in WB and EIA. Suitable for Human and Mouse.

Quick Overview for TDGF1 antibody (Internal Region) (ABIN401391)

Target: TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TDGF1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-TDGF1 Antibody

Binding Specificity: Internal Region

Specificity: This antibody is specific for Cripto-1 protein. Expect cross-reactivity with Cripto-3 (TDGF2) based on very high levels of sequence conservation.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to an internal sequence of human Cripto-1 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/50,000 - 1/150,000. Western Blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.80 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for TDGF1

Target: TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))

Alternative Name: TDGF1 / CRIPTO

Background: Human Cripto-1 (CR-1), also known as Teratocarcinoma-derived growth factor 1 (TDGF1), is a member of the epidermal growth factor (EGF)-GFC family and has been implicated in both embryogenesis and carcinogenesis. During early vertebrate development, CR-1 functions as a co-receptor for Nodal, a transforming growth factor beta (TGFbeta) family member, and is essential for mesoderm and endoderm formation and anterior-posterior and left-right axis establishment. In adult tissues, CR-1 is expressed at a low level in all stages of mammary gland development, and expression increases during pregnancy and lactation. Over-expression of CR-1 in mouse mammary epithelial cells leads to their transformation in vitro, and when injected in mammary glands, CR-1 produces ductal hyperplasias.Synonyms: CRGF, Cripto-1 growth factor, Epidermal growth factor-like cripto protein CR1, Teratocarcinoma-derived growth factor 1

Gene ID: 6997

NCBI Accession: NP_001167607

UniProt: P13385

Pathways: EGFR Signaling Pathway