PPP1R13B antibody (Internal Region)

Catalog No. ABIN401394

The Rabbit Polyclonal anti-PPP1R13B antibody (ABIN401394) specifically detects PPP1R13B in WB and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for PPP1R13B antibody (Internal Region) (ABIN401394)

Target: PPP1R13B (Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R13B antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PPP1R13B Antibody

Binding Specificity: Internal Region

Specificity: This antibody is directed against ASPP1.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to an internal sequence of human ASPP1

Isotype: IgG

Application Details

Application Notes: ELISA: 1/2,500 - 1/10,000. Western Blot: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.3 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for PPP1R13B

Target: PPP1R13B (Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B))

Alternative Name: PPP1R13B

Background: ASPP (ankyrin-repeat-, SH3-domain- proline-rich-region protein) proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, iASPP functionally inactivates p53. ASPPs may also regulate p63- and p73-mediated apoptosis. Both ASPP1 and 2 directly interact with p53 and specifically enhance the apoptotic function of p53 by stimulating its DNA binding and transactivation function on promoters of pro-apoptotic genes, such as Bax and PIG-3. Not all cell cycle arrest genes are affected, such as p21. Interestingly, expression of ASPP is frequently down-regulated in human breast carcinomas expressing wild-type p53 but not mutant p53. Therefore, ASPP might regulate the tumor suppression function of p53 in vivo.Synonyms: ASPP1, Apoptosis-stimulating of p53 protein 1, KIAA0771, Protein phosphatase 1 regulatory subunit 13B

Gene ID: 23368

NCBI Accession: NP_056131

UniProt: Q96KQ4

Pathways: p53 Signaling