Keratin Basic (AA 266-299) antibody

Catalog No. ABIN4219252

This Mouse Monoclonal antibody specifically detects Keratin Basic in WB, IHC (fro) and IHC (fp). It exhibits reactivity toward Human. It has been mentioned in 7+ publications

Quick Overview for Keratin Basic (AA 266-299) antibody (ABIN4219252)

Target: Keratin Basic

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: AE3

Product Details

Binding Specificity: AA 266-299

Specificity: Most basic (type II) keratins

Cross-Reactivity: Cow (Bovine), Chicken, Human, Rat (Rattus), Mouse (Murine), Rabbit, Monkey

Purification: Ion-exchange chromatography

Immunogen: Human epidermal keratin

Isotype: IgG1

Application Details

Application Notes: IHC: 1:100

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: reconstitue with 1 mL deionized water

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.4, 0.1 % sodum azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C

References

Waseem, Karsten, Leigh, Purkis, Waseem, Lane: "Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability." in: Biochemistry, Vol. 43, Issue 5, pp. 1283-95, (2004) (PubMed).

Chesa, Rettig, Melamed: "Expression of cytokeratins in normal and neoplastic colonic epithelial cells. Implications for cellular differentiation and carcinogenesis." in: The American journal of surgical pathology, Vol. 10, Issue 12, pp. 829-35, (1986) (PubMed).

Nomori, Kameya, Shimosato, Saito, Ebihara, Ono: "Nasopharyngeal carcinoma: immunohistochemical study for keratin, secretory component and leukocyte common antigen." in: Japanese journal of clinical oncology, Vol. 15, Issue 1, pp. 95-105, (1985) (PubMed).

Weiss, Eichner, Sun: "Monoclonal antibody analysis of keratin expression in epidermal diseases: a 48- and 56-kdalton keratin as molecular markers for hyperproliferative keratinocytes." in: The Journal of cell biology, Vol. 98, Issue 4, pp. 1397-406, (1984) (PubMed).

Battifora, Sheibani, Tubbs, Kopinski, Sun: "Antikeratin antibodies in tumor diagnosis. Distinction between seminoma and embryonal carcinoma." in: Cancer, Vol. 54, Issue 5, pp. 843-8, (1984) (PubMed).

Woodcock-Mitchell, Eichner, Nelson, Sun: "Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies." in: The Journal of cell biology, Vol. 95, Issue 2 Pt 1, pp. 580-8, (1983) (PubMed).

Tseng, Jarvinen, Nelson, Huang, Woodcock-Mitchell, Sun: "Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies." in: Cell, Vol. 30, Issue 2, pp. 361-72, (1983) (PubMed).

Target Details

Target: Keratin Basic

Background: AE 3 represents an excellent marker fort distinguishing carcinoma from non-epithelial tumors. Tumors specifically detected: All epithelia and their neoplasms.

Molecular Weight: 53 - 67 kDa