Calretinin antibody

Catalog No. ABIN452385

Product Details anti-Calretinin Antibody

Target: Calretinin (CALB2) (Calbindin 2 (CALB2))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calretinin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Specific for Calretinin. No cross-reaction with Calbindin and Calmodulin. The antibody is suitable for the detection of Calretinin in neurons and may be used as an aid in differential diagnosis of pleura-mesothelioma. This marker is frequently expressed in epitheloid mesothelioma, while occasionally it turns positive in sarcomatous mesothelioma and carcinoma. It was found to be negative in sarcoma (2,3) and in 90 % of adenocarcinoma.

Cross-Reactivity (Details): Species reactivity (tested):Human and Rat.

Characteristics: Synonyms: CALB2, CAB29, 29 kDa calbindin

Immunogen: Recombinant Human Calretinin 22kDa.

Clone: 6B3

Isotype: Ig Fraction

Application Details

Application Notes: Immunohistochemistry on Frozen and Paraffin Sections. (Improvement of staining withUnmasking Fluid G or Unmasking Fluid C)Immunoblotting. (1/40-1/50)Positive Control: Cerebral cortex, pleural mesothelioma. Incubation Time: 60 min at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Stabilized Phosphate buffer, pH 7.3 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Calretinin

Target: Calretinin (CALB2) (Calbindin 2 (CALB2))

Alternative Name: Calretinin (CALB2 Products)

Synonyms: CAB29 antibody, CAL2 antibody, CR antibody, calb2l antibody, wu:fq18e08 antibody, zgc:73115 antibody, calb2 antibody, wu:fq17g09 antibody, zgc:73099 antibody, calbindin 2 antibody, calbindin 2a antibody, calbindin 2b antibody, CALB2 antibody, Calb2 antibody, calb2a antibody, calb2b antibody

Background: Calretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98 % sequence homology and 91 % homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum. The antibodies against calretinin also labels the mossy fibers throughout, and climbing fibers in a distinct group of folia. They also stain several cell types in the molecular layer. There is no appreciable immunostaining of calretinin in cerebellar cortex. In the rat, antibodies against calretinin stain the Lugaro cells, and some granule cells in lobe X, they also give weak staining of all the granule cells in the other lobes. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc.Synonyms: 29 kDa calbindin, CAB29, CALB2

Gene ID: 794

UniProt: P22676