EGF antibody (HRP)

Catalog No. ABIN452468

The Mouse Monoclonal anti-EGF antibody is suitable to detect EGF in samples from Human. It has been validated for EIA.

Quick Overview for EGF antibody (HRP) (ABIN452468)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGF antibody is conjugated to HRP

Application: Enzyme Immunoassay (EIA)

Clone: S-146

Product Details anti-EGF Antibody (HRP)

Specificity: Reactive with natural and recombinant Human EGF. Does not cross react with other Human cytokines tested such as IL-1beta, IL-8, MCAF, TGF-beta and SAA.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: Urogastrone, Epidermal growth factor, URG, HOMG4, Pro-epidermal growth factor

Purification: Affinity Chromatography on Protein G

Immunogen: Purified recombinant human EGF, 6kDa, 53 amino acid residues.

Isotype: IgG1

Application Details

Application Notes: ELISA: This HRP-conjugated Monoclonal EGF antibody can be used as a tracer/detectionantibody in a Sandwich ELISA for Human EGF detection in combination with a Captureantibody (Cat#DM1012). Suggested Capture Coating Dose: 0.3 μg/well. Substrate: TMB. If the above suggested conditions are followed approximately 20 pg/mL ofEGF in serum/plasma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M PBS, pH 7.0 ± 0.1 in 50 % Glycerol and 0.01 % Thimerosal as a bacteriostat

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF

Background: Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone

Gene ID: 1950

UniProt: P01133

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation