Endothelin-1 Receptor antibody (AA 132-162)

Catalog No. ABIN452933

The Rabbit Polyclonal anti-Endothelin-1 Receptor antibody is suitable to detect Endothelin-1 Receptor in samples from Human. It has been validated for WB, IF, FACS and EIA.

Quick Overview for Endothelin-1 Receptor antibody (AA 132-162) (ABIN452933)

Target: Endothelin-1 Receptor (EDNRA) (Endothelin Receptor Type A (EDNRA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endothelin-1 Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Product Details anti-Endothelin-1 Receptor Antibody

Binding Specificity: AA 132-162

Specificity: This antibody detects Human Endothelin A receptor at Center. Other species not tested.

Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide selected between aa 132-162 amino acids from the Center region of Human EDNRA

Application Details

Application Notes: Western blot: 1: 50 - 1: 100. ELISA: 1: 1,000. Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS, 0.09 % (W/V) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Endothelin-1 Receptor

Target: Endothelin-1 Receptor (EDNRA) (Endothelin Receptor Type A (EDNRA))

Alternative Name: Endothelin A Receptor

Background: EDNRA is a receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Function: Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3 Cellular Location: Cell membrane, Multi-pass membrane protein. Tissue Location: Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels.Synonyms: EDNRA, ET-A, ETA, ETA-R, ETRA, Endothelin-1 receptor, endothelin receptor type A, hET-AR

Molecular Weight: 48722 Da

Gene ID: 1909

NCBI Accession: NP_001948

UniProt: P25101

Pathways: cAMP Metabolic Process