Endothelin-1 Receptor antibody (Extracellular)

Catalog No. ABIN7581867

The Rabbit Polyclonal anti-Endothelin-1 Receptor antibody (ABIN7581867) specifically detects Endothelin-1 Receptor in WB, FACS and LCI. The antibody is reactive with Rat samples.

Quick Overview for Endothelin-1 Receptor antibody (Extracellular) (ABIN7581867)

Target: Endothelin-1 Receptor (EDNRA) (Endothelin Receptor Type A (EDNRA))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endothelin-1 Receptor antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-Endothelin-1 Receptor Antibody

Binding Specificity: AA 329-341, Extracellular

Purpose: A Rabbit Ployclonal Antibody to Endothelin Receptor A

Sequence: KKTVYDEMDK NRC

Specificity: Extracellular, 3rd loop.

Predicted Reactivity: Mouse - identical Human - 12 out of 13 amino acid residues identical

Characteristics: Anti-Endothelin Receptor A (extracellular) Antibody (ABIN7581867) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize ET-A Receptor from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: KKTVYDEMDKNRC, corresponding to amino acid residues 329 - 341 of rat EDNRA

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:400

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Endothelin-1 Receptor

Target: Endothelin-1 Receptor (EDNRA) (Endothelin Receptor Type A (EDNRA))

Alternative Name: EDNRA

Background: EDNRA, ET-A, ET-AR, ETRA, ET1-Specific type endothelin receptor, Endothelin-1 receptor, Endothelin Receptor Type A,The endothelin system is comprised of three active peptides, ET-1, 2, and 3, which are considered to be very powerful vasoconstrictive substances. In humans, endothelins mediate their actions via two specific G-Protein Coupled Receptors, ETAR and ETBR. Both ETAR and ETBR are present in heart and in human myocardium at similar levels.1,2The endothelin receptors differ in their ligand specificity. While ETAR has varying affinities for the endothelin isoforms (ET-1 >ET-2>ET-3), ETBR shows no selective affinity.2,3 Subsequent studies have demonstrated the presence of endothelins in vascular as well as in non-vascular cells and tissues, having multiple biological activities.Currently, there is increasing evidence that ET-1 may modulate mitogenesis, apoptosis, angiogenesis tumor invasion and the development of metastases.3Overexpression of ET-1 and ETAR was reported in different malignancies including prostate cancer human Kaposi's sarcoma, ovarian and breast carcinomas.4In breast carcinomas overexpression of ET-1 and ETA receptors correlated with parameters that characterize aggressive types of breast cancer suggesting that analysis of ETAR expression might be used as a diagnostic marker for evaluating the progression of the disease and effectiveness of treatment. These and other findings have made ET receptors, and especially ETAR, promising therapeutic targets for pharmacological intervention.5-9

Gene ID: 24326

UniProt: P26684

Pathways: cAMP Metabolic Process