Endothelin-1 Receptor antibody (Intracellular)

Catalog No. ABIN7043119

This Rabbit Polyclonal antibody specifically detects Endothelin-1 Receptor in WB, IF, IHC and IC. It exhibits reactivity toward Rat.

Quick Overview for Endothelin-1 Receptor antibody (Intracellular) (ABIN7043119)

Target: Endothelin-1 Receptor (EDNRA) (Endothelin Receptor Type A (EDNRA))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endothelin-1 Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunochromatography (IC)

Product Details anti-Endothelin-1 Receptor Antibody

Binding Specificity: AA 413-426, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Endothelin Receptor A

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,swine,14 amino acid residues identical,bovine - 13, chicken - 12

Characteristics: Anti-Endothelin Receptor A Antibody (ABIN7043119, ABIN7044238 and ABIN7044239) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize ET-AR from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)NHNTERSSHKDSMN, corresponding to amino acid residues 413-426 of rat ET-A

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC|ICC

Negative Control: (ABIN7235266)

Blocking Peptide: (ABIN7235266)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Endothelin-1 Receptor

Target: Endothelin-1 Receptor (EDNRA) (Endothelin Receptor Type A (EDNRA))

Alternative Name: EDNRA

Background: EDNRA, ETA, ET-AR, ETRA, ET1-Specific type endothelin receptor, Endothelin-1 receptor,The endothelin system is comprised of three active peptides, ET-1, 2, and 3, which are considered to be very powerful vasoconstrictive substances. In humans, endothelins mediate their actions via two specific G-Protein Coupled Receptors, ETAR and ETBR. Both ETAR and ETBR are present in heart and in human myocardium at similar levels.1,2The endothelin receptors differ in their ligand specificity. While ETAR has varying affinities for the endothelin isoforms (ET-1 >ET-2>ET-3), ETBR shows no selective affinity.2,3 Subsequent studies have demonstrated the presence of endothelins in vascular as well as in non-vascular cells and tissues, having multiple biological activities.Currently, there is increasing evidence that ET-1 may modulate mitogenesis, apoptosis, angiogenesis tumor invasion and the development of metastases.3Overexpression of ET-1 and ETAR was reported in different malignancies including prostate cancer human Kaposi's sarcoma, ovarian and breast carcinomas.4In breast carcinomas overexpression of ET-1 and ETA receptors correlated with parameters that characterize aggressive types of breast cancer suggesting that analysis of ETAR expression might be used as a diagnostic marker for evaluating the progression of the disease and effectiveness of treatment. These and other findings have made ET receptors, and especially ETAR, promising therapeutic targets for pharmacological intervention.5-9

Alternative names: Endothelin Receptor A, EDNRA, ETA, ET-AR, ETRA, ET1-Specific type endothelin receptor, Endothelin-1 receptor

Gene ID: 24326

NCBI Accession: NM_001166055

UniProt: P26684

Pathways: cAMP Metabolic Process