HADHA antibody (C-Term)

Catalog No. ABIN453085

This Rabbit Polyclonal antibody specifically detects HADHA in WB, FACS, IHC (p) and EIA. It exhibits reactivity toward Human and Mouse.

Quick Overview for HADHA antibody (C-Term) (ABIN453085)

Target: HADHA (Hydroxyacyl-Coenzyme A Dehydrogenase/3-Ketoacyl-Coenzyme A Thiolase/enoyl-Coenzyme A Hydratase (Trifunctional Protein), alpha Subunit (HADHA))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HADHA antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-HADHA Antibody

Binding Specificity: AA 734-763, C-Term

Specificity: This antibody recognizes Human and Mouse HADHA (C-term).

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Purification: Protein A Chromatography, followed by peptide affinity purification

Immunogen: KLH conjugated synthetic peptide between 734~763 amino acids from the C-terminal region of human HADHA

Application Details

Application Notes: ELISA: 1/1,000Western blotting: 1/100approx. 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HADHA

Target: HADHA (Hydroxyacyl-Coenzyme A Dehydrogenase/3-Ketoacyl-Coenzyme A Thiolase/enoyl-Coenzyme A Hydratase (Trifunctional Protein), alpha Subunit (HADHA))

Alternative Name: HADHA

Background: Mitochondrial Trifunctional Protein (TFP) is a multienzyme complex of the beta-oxidation cycle. TFP deficiency is a clinically heterogeneous disorder with phenotypes of different severity. The spectrum of diseases range from severe neonatal/infantile cardiomyopathy and early death to mild chronic progressive sensorimotor poly-neuropathy with episodic rhabdomyolysis. Human TFP is an octomer composed of four alpha-subunits and four beta-subunits. Mutations in either subunits may result in general TFP deficiency with reduced activity of all enzymes. HADHA is the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Synonyms: HADH, Long chain 3-hydroxyacyl-CoA dehydrogenase, Long-chain enoyl-CoA hydratase, TP-alpha, Trifunctional enzyme subunit alpha mitochondrial

Gene ID: 3030

NCBI Accession: NP_000173

UniProt: P40939

Pathways: Monocarboxylic Acid Catabolic Process