PRAME antibody (C-Term)

Catalog No. ABIN453177

The Rabbit Polyclonal anti-PRAME antibody (ABIN453177) specifically detects PRAME in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for PRAME antibody (C-Term) (ABIN453177)

Target: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRAME antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PRAME Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to MAPE.

Purification: Saturated Ammonium Sulfate (SAS) precipitation

Immunogen: KLH conjugated synthetic peptide selected from the C-terminal region of human MAPE

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PRAME

Target: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Alternative Name: PRAME / MAPE

Background: MAPE is an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, its gene is also expressed in acute leukemias.Synonyms: Melanoma antigen preferentially expressed in tumors, OIP-4, OIP4, OPA-interacting protein 4, Preferentially expressed antigen of melanoma

Gene ID: 23532

NCBI Accession: NP_006106

UniProt: P78395

Pathways: Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding