PABP antibody (Middle Region)

Catalog No. ABIN453247

This Rabbit Polyclonal antibody specifically detects PABP in WB and EIA. It exhibits reactivity toward Human and Mouse.

Quick Overview for PABP antibody (Middle Region) (ABIN453247)

Target: PABP (PABPC1) (Poly(A) Binding Protein, Cytoplasmic 1 (PABPC1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PABP antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PABP Antibody

Binding Specificity: Middle Region

Specificity: This antibody reacts to PABPC1.

Purification: Saturated Ammonium Sulfate (SAS) precipitation

Immunogen: KLH conjugated synthetic peptide selected from the Center region of human PABPC1

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PABP

Target: PABP (PABPC1) (Poly(A) Binding Protein, Cytoplasmic 1 (PABPC1))

Alternative Name: PABPC1

Background: PABPC1 binds the poly(A) tail of mRNA. It may be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. PABPC1 can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. It may be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.Synonyms: PAB1, PABP-1, PABP1, PABPC2, Poly(A)-binding protein 1, Polyadenylate-binding protein 1

Gene ID: 26986

NCBI Accession: NP_002559

UniProt: P11940

Pathways: SARS-CoV-2 Protein Interactome