NOD1 antibody (C-Term)

Catalog No. ABIN453551

The Rabbit Polyclonal anti-NOD1 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect NOD1 in samples from Human.

Quick Overview for NOD1 antibody (C-Term) (ABIN453551)

Target: NOD1 (Nucleotide-Binding Oligomerization Domain Containing 1 (NOD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-NOD1 Antibody

Binding Specificity: C-Term

Specificity: This antibody recognizes CARD4 / NOD1 (C-term).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A.

Immunogen: KLH conjugated synthetic peptide selected from the C-terminal region of Human CARD4. Genename: NOD1

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NOD1

Target: NOD1 (Nucleotide-Binding Oligomerization Domain Containing 1 (NOD1))

Alternative Name: CARD4 / NOD1

Background: CARD4 is a member of the NOD (nucleotide-binding oligomerization domain) family. This member is a cytosolic protein. It contains an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and 10 tandem leucine-rich repeats (LRRs) in its C terminus. The CARD is involved in apoptotic signaling, LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. This protein is an intracellular pattern-recognition receptor (PRR) that initiates inflammation in response to a subset of bacteria through the detection of bacterial diaminopimelic acid.Synonyms: Caspase recruitment domain-containing protein 4, Nucleotide-binding oligomerization domain-containing protein 1

Molecular Weight: 107691 Da

Gene ID: 10392

NCBI Accession: NP_006083

UniProt: Q9Y239

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades, Inflammasome